Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLMERNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNEHNAKMGLVCA-------TPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIA----NNASISGAEGGCQAEV-GSASAMSAAALTLAA--------------GGTPYQASQAIAFVIKNMLGL----ICDPVAGLVEVPCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLATTPTGRRLQKEIFGE 5N5X Chain:C ((1-440)) MFEVPITLTNRKFAQRRKLKYQYINYISRRFDRISKQQKKRRRERHWR----SVSFEIWRTVSSQNKQPINKQKMT--YHNFKKIEKIPLRKMEIPLLHCTKENKLYFQSISRGLEPLKTSTSEVRNYRTRHIVTLTDLLHLNVSRH-NWSLAYKIFATLIRIPGVQIKSLWGIGVEILDNLSNSSSGLDFLQWMCQIYSSKSRSIVPPFQTGSRTHTAKFAITYLWSSLINCQKSMLIDKISEWVLTPPFMEDAE-VWFIYASCHLLKADINQVIKHIHYVRTFLKICLD---------KGGFAVPSRLIENQLKSFES

General information: TITO was launched using: RESULT: Template: 5N5X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 823 -20383 -24.77 -74.66 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain C : 0.64 3D Compatibility (PKB) : -24.77 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.177

(partial model without unconserved sides chains): PDB file : Tito_5N5X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5N5X-query.scw PDB file : Tito_Scwrl_5N5X.pdb: