Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---MESRLMKNKRYFFDTILIILLLISTIFCVSPVFIKLDILGTPSHAILTFVLAIPL-----FYILSQCLHTLLLL------------VSSIFCKLRPIYFYFIFVII-------IGARKYYRILFHQLMGFSPGIA-------VFYKES-----QTTKNL----FKFYYFLYFTTLISYYFFFTFVYDKPLLL-----------PLISFSIIIALVQKLY------------------------------------RIENQQLFLLK---------------------SKVLTIL---------ESKKNCEFNLQDYHEIWKLQSKSELPCVALSYISLIKPYLSESVREQIDLLEVKRFKKINHPISLYGMLDVIKLNLY---------LRHYNEKNKYESMLKKIL-EVRPDFVLIEQNIDDSLNSS---QPLSLSLAISEIQLLLEVYMGIKHVSIRR--------------------- 4K70 Chain:A ((2-483)) PGSMEALVRALEEADHAVATVVQSRILEFFMAAGRETPAGVRGLWARALRLACRAYVETGTCEAAVLAENLAGLALWRLRHDWDEGTAPLLELLGVVNGDDTTAALTEAGLRTSAEFGPDAMFRLVSEWCAAFDEALAGARSADDVLAAPRVVPPEQTARALVQPRFATLYDMDFVQDGLRYVAQHTNWALPLALAVRQMQNEGLKPLTRALFALTIADEFFHDRQNPTLREQFAEAARAVDEAALVPVGEVNATPRTAVEVRVSAALAHGDAYVRELRPGTVARRLRTDQGVLALLDPGAQAVHVAAAADLDHTQVDATGVWEAVQASASPLQVVE--ALVTAGFTRRHCDLLERAVLDRAPRLTDAQRAVGCTAVVGGVVHRLLDDYGPGLDYVRAYTDVADTLEPLYGDVTAALGLPEKGVEHVVRHCMAPRPPTEHVGAARAALLREVAAAERRAGLAHSAAREALNTWLAFRAQSRWGL

General information: TITO was launched using: RESULT: Template: 4K70.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1484 -80610 -54.32 -245.76 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -54.32 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.237

(partial model without unconserved sides chains): PDB file : Tito_4K70.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4K70-query.scw PDB file : Tito_Scwrl_4K70.pdb: