Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------------------------------------------------------------------------------------------------------------------MKKMK-EVKFHLATGLLI----LTYYL-IFNVTSDLDFMVAL-SDNMYYVFQVLLVLILGTIATIAFVKSEHWKECGRFQFRWSYLGVFLLSFFLLFVWANLTTYIF------PRTQNGSTV--------VEVATSLTG-ISYFVTRI-LYTSIIAPVSE--EVVCRGLLMTSLSKVNRYYLDVLVSAAIFGAMHVLQYGWITTDFIKYFGMGLIFCMMFRYTRSIYWAIALHASWNSFLLIVTLLVFGY--------------------------------- 4OL8 Chain:A ((24-461)) PVWLQQKYREIIRNDLPPPVKHDIEIKPGARLPRLQPYHVTEKNEQEINKIVQKLLDNKFIVPSKSPCSSPVVLVPKTFRLCVDYRTLNKATISDPFPLPRIDNLLSRIGNAQIFTTLDLHSGYHQIPMEPKDRYKTAFVTPSGKYEYTVMPFGLVNAPSTFARYMADTFRDL--RFVNVYLDDILIFSESPEEHWKHLDTVLERLKNENLIVKKKKCKFEETEFLGYSIGIQKIAPLQHKCAAIRDFPTPKTVKQAQRFLGMINYYRRFIPNCSKIAQPITEKQDKAIDKLKDAPFNNKANYRLTTDASK-----------DGIGAVLEEVDNKNKLVGVVGYFSKSLEYP---AGELELLGIIKALHHFRYMLHGKHFTLRTNHARRVQRWLDDLATYDFTLEY

General information: TITO was launched using: RESULT: Template: 4OL8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 898 -53187 -59.23 -254.48 target 2D structure prediction score : 0.26 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -59.23 2D Compatibility (Sec. Struct. Predict.) : 0.26 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.011

(partial model without unconserved sides chains): PDB file : Tito_4OL8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4OL8-query.scw PDB file : Tito_Scwrl_4OL8.pdb: