Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLEAVHTEKQWERSATELPPFIIKRLPVRLTFDNNYFNDRYQVIPIGGYNVIIENILKDVEVGLGVDCFDYREELEASANKVAFTGMIDQYFDYKHGELEYRSLRFKHEVLDEENHQGNAVVNYTDREIPYTRIIEHKKFE-YGLQPKTVITHEYPADWKRGDEPYYPINDEKNNAIFSKYHEEASQNDKVIFCGRLADYKY 3INT Chain:A ((155-349)) -----YTIKQWGMQPSELPASILKRLPVRFNYDDNYFNHKFQGMPKCGYTQMIKSILNHENIKVDLQ-REFIVDERTHYDHVFYSGPLDAFYGYQYGRLGYRTLDFK-KFIYQGDYQGCAVMNYCSVDVPYTRITEHKYFSPWEQHDGSVCYKEYSRACEENDIPYYPIRQMGEMALLEKYLSLAENETNITFVGRLGTYRY

General information: TITO was launched using: RESULT: Template: 3INT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 562 -14276 -25.40 -73.59 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -25.40 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.349

(partial model without unconserved sides chains): PDB file : Tito_3INT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3INT-query.scw PDB file : Tito_Scwrl_3INT.pdb: