Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDLRYL-IDTEEDKLNQTRYTQPAILATSVAIY-RLLQEKGYQPDMVAGLSLGEYSALVASGALDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYNTPAQIVIAGEVVAVDRAVELLQE-AGAKRLIPLKVSGPFHTSLLEPASQKLAETLAQVSFSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGKVLSGFVKKIDQTAHLAHVEDQASLVALLEK 5DZ6 Chain:A ((4-283)) -----YVFPGQGSQKQGMGSGLFDEF---KELTDQADEILGYSIKRLCLENPYSNLNKTQFTQPALYVVNALSYLKKIRDEEVKPDFVAGHSLGEYNALFAAEAFDFETGLQLVRKRGELM---SLISNGGMAAVMGLNEEQVAKAL-KEYHLHDVDIANVNAPYQIVISGKKDEIEKAASLFETMTEVTMVLPLNVSGAFHSRYMNKAKEEFEEFLHAFYFSPPSIPVISNVYAKPYTYEFMKQTLADQINHSVKWTDSISYLMKKAAMEFEEVGPGNVLTGLIHRIKKDA-----------------

General information: TITO was launched using: RESULT: Template: 5DZ6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1537 -8071 -5.25 -29.14 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -5.25 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.593

(partial model without unconserved sides chains): PDB file : Tito_5DZ6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DZ6-query.scw PDB file : Tito_Scwrl_5DZ6.pdb: