Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNKFFLIAILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDHAFGQTILDKKPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKELNGKANLFDDLDGLNFEAISNSKPDVILAG---YSGITKEDYDTLSKIAPVAAYKSKPWQTLWRDMI---KIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAADTSKFWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAK----EISAEEANKINDADVIITYG----DDKTLEALQKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLGNACKNAK 3LHS Chain:A ((5-266)) ------------------------------------ASTISVKDENGTVKVPKDAKRIVVLEYSFADALAALDVKPVGI--------ADDGKKKRIIKPVREKIGDYTSVGTRKQPNLEEISKLKPDLIIADSSRHKGINKE----LNKIAPTLSLKS--FDGDYKQNINSFKTIAKALNKEKEGEKRLAEHDKLINKYKD---EIKFD-RNQKVLPAVVAKAGLLAHPNYSY---VGQFLNELGFKNALSDDVTKGLSKYLKGPYLQLDTEHLADLNPERMIIMTDHAKKDSAEFKKLQEDATWKKLNAVKNNRVDIV------------------------------------

General information: TITO was launched using: RESULT: Template: 3LHS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1209 1960 1.62 7.90 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 1.62 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.548

(partial model without unconserved sides chains): PDB file : Tito_3LHS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3LHS-query.scw PDB file : Tito_Scwrl_3LHS.pdb: