Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------------------------------------------------------------MGVVGKQSSSFS--LLFWLFSLSSGKVTCFKSGSLGNSSNILPKLSHFVNFTAIGVPRF--------------------------------- 4M91 Chain:A ((2-154)) SKKENLLAEKVEQLMEWSSRRSIFRMNGDKFRKFIKAPPRNYSMIVMFTALQPQRQCSVSRQANEEYQILANSWRYSSAFSNKLFFSMVDYDEG-TDVFQQLNMNSAP-------TFMHFPPKGRPKRADTFDLQRIGFAAEQLAKWIADRTDVHIRVFRL

General information: TITO was launched using: RESULT: Template: 4M91.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 79 -4079 -51.63 -83.23 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -51.63 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.097

(partial model without unconserved sides chains): PDB file : Tito_4M91.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4M91-query.scw PDB file : Tito_Scwrl_4M91.pdb: