Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTVEEEKAFLARHLKA--TEA-------GEFVTIDALFQAYKKELGRSYTRDAFYQLLKRHGWRNITPRPEHPRKADAQTIVASKNKISIQEDKKAL 4ZDT Chain:D ((12-68)) ---------ISQVVKQAKDNSVWIKILTYSAIDVEEFQLWLKRK-NLNVSLDLIKSWCDKYGVLMKG------------------------------

General information: TITO was launched using: RESULT: Template: 4ZDT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 124 -8330 -67.18 -173.54 target 2D structure prediction score : 0.81 Monomeric hydrophicity matching model chain D : 0.61 3D Compatibility (PKB) : -67.18 2D Compatibility (Sec. Struct. Predict.) : 0.81 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.742

(partial model without unconserved sides chains): PDB file : Tito_4ZDT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZDT-query.scw PDB file : Tito_Scwrl_4ZDT.pdb: