Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGIVDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAFKQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVEKFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDIMTNHINS-GMSKMFESLLITLALGAGTSVALVLMN 4D10 Chain:H ((14-212)) FSFKKLLDQCENQELEA-----------------------PGGIATPPVYGQLLALYLL--HNDMNNARYLW------------KRIPPAIKSANSELGGIWSVGQRIWQRDFPGIYTTIN----------AHQWSETVQPIMEALRDATRRRAFALVSQAYTS--------------IIADDFAAFVGLP-------------------VEEAVKGILEQGWQADSTTRMVLPRKPEQQLARLTDYVAFLEN

General information: TITO was launched using: RESULT: Template: 4D10.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain H - contact count / total energy / energy per contact / energy per residue : 593 -101609 -171.35 -590.75 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain H : 0.59 3D Compatibility (PKB) : -171.35 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.325

(partial model without unconserved sides chains): PDB file : Tito_4D10.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4D10-query.scw PDB file : Tito_Scwrl_4D10.pdb: