Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MWIAISALNLAFAVMLGAFGAH-----------------------GLKAHASPEQLAWWQTA----TDYFFYHALGLLALGILSKVLPHFPIKLSFLLIQIGILFFCGSLYIMALGLPRILGAITPIGGALMIAGWLILAWNAFKYAK 2AO7 Chain:A ((29-192)) CCYKLKPGKQCSPSQGPCCTAHCAFKSKTEKCRDDSDCAKEGICNGITALCPASDPKPNFTDCNRHTQVCINGQCAGSICEKHGLEECTCKELCHVCCMKKMEPSTCASTGSVQWNKYFLGRTITLQPGSPC--NDFRGYCDVFMRCR

General information: TITO was launched using: RESULT: Template: 2AO7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 490 -56175 -114.64 -472.06 target 2D structure prediction score : 0.20 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -114.64 2D Compatibility (Sec. Struct. Predict.) : 0.20 1D Compatibility (Hydrophobicity) : 0.62 QMean score : -0.124

(partial model without unconserved sides chains): PDB file : Tito_2AO7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2AO7-query.scw PDB file : Tito_Scwrl_2AO7.pdb: