Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRQGFIKAFANLVGWIFALIMGAKYAVLLAPSMSGLSQDPVVQKIAAFAFIALLIIVLTWIVTAFLNGLLKSLKLGPLNRLAGGAFGSLKGLLVVLITMQGVGPWVESSPHWKQSKFIQFLLPYAPLATELSKDAASEAFHQITSGGGVTRTSPKPMDESEETELRPDHSTKDPFY 5TKZ Chain:A ((4-92)) -----------------------------------------------------------QVRTLFVSGLPMDAKPRELYLLFRGARG-YEGALLKMTSKNG-----------KPTSPVGFVTF-------LSQQDAQDARKMLQG----VRFDPEAAQVLRLELAKSNTKV-----

General information: TITO was launched using: RESULT: Template: 5TKZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 381 -35755 -93.84 -401.74 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.52 3D Compatibility (PKB) : -93.84 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.52 QMean score : 0.384

(partial model without unconserved sides chains): PDB file : Tito_5TKZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5TKZ-query.scw PDB file : Tito_Scwrl_5TKZ.pdb: