Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPIDTQLTTLFCLIDDFCTDITQNVEQYMLTYGQTKRLRQSKIHASEVITLLLWFHLTGSRNFKAFYLYWAKPFLCSYFTNLPSYSRFIELKAKYAMYFVALIESLKVHSAGIAFIDSTKLAVCHNKRIHQHRVFADSASRGKTSVDWFYGFKIHLICDHIGRLVSYCITTGNVDDRKVLPDLIEHSKLKGKLFGDRGYVGKNWKSRLAEVGVQLITRVKRNMKPQVLAPFDHAVLKKRGIIEAPFKLMKSQFDLEHSRHRSKMGLLTTIFAALTLYALVLVNGYKSGIQQILKPIDLNST 2E74 Chain:E ((1-32)) -------------------------------------------------------------------------------------------MILGAVFYIVFIALFFGIAVGIIFAIKSIKLI----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2E74.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 20 -4903 -245.13 -153.20 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain E : 0.49 3D Compatibility (PKB) : -245.13 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.49 QMean score : 0.120

(partial model without unconserved sides chains): PDB file : Tito_2E74.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2E74-query.scw PDB file : Tito_Scwrl_2E74.pdb: