Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------MHEITLHEVRQLIASLRTVYAAQFNKQFPTSGESAIPLSV-VEQIALKT-----LVGVQQNQFNNALARLLTAGGRFM-------------PSF-AEFRTWCIGESWMSPE--EAWSRACKFTTDRTVVITQITKYALDEV-MYLIEAGQMRAAQDNF-FGTYNVMVAK---AQLKGRQQEFYTPPLQLEHKEPEHTPVSNDEVQK---------QLKSLMERLKINGRKPAPVQKLQAKE-KEPELA-KELGPDPFDNPHEYAEMCRREGMPIPRNILQLIEGANV------------ 5KLP Chain:A ((18-342)) TQSMRLQQKINDLKPYVRHARGPIKAYGQAALDRASGAVSFAELDATHLDAMVYIENQRNPGLNLKHFRDHYYLIQALQSDGPSAFRAIFPQTCPETGQTLKHHVMADVRLHQGAPTIIITEPAVIVGARYQQLQRHNLTLEDLSESGVPLSQVAIIETQAAATSDDCVMYSLNYAIKAHKNAAQFDDIHHGLQHGTLSTESESRARTTLGALEASSSYSVMHEGAHAAFGADVLPVDFYKHGASLTQAYYLMKRPDGRMAGRVNSEGHSEAENLVQ-RNQAFRVKTQFSASIDGFRLQEIKRVLAAAQR

General information: TITO was launched using: RESULT: Template: 5KLP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1196 34296 28.68 138.29 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 28.68 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.077

(partial model without unconserved sides chains): PDB file : Tito_5KLP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5KLP-query.scw PDB file : Tito_Scwrl_5KLP.pdb: