Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMLTSKASLRLTLLASAIFLVACQPKSDPKESEDQQKPAVVEQKPVELTLKGETVPSKVTLPDCDGKTCPEFTVARLQSNFPFIDKIIDQQVLKALGQILEIAEPDAKAAQADKKTEASTAAATEQQDSFDAQVQRYANSFIDLDNDLKALSSNHQINLLVKPKIIQSQGKVVTVVVNSSSYLGGAHGSAAQQYYNFDLKKEKQVKLEGLLRPEKKAALEKLAHEAFKAWVTDSKLVNSVSEYEQAWPFKLTENFLLGDQGLILQYGEYEIGPYVVGLPRLVIPYDQLQDVLKEEYLPQPKAKPASTPAVKSTS 3CYG Chain:B ((103-238)) ---------------------------------------------------------------------------------------------------------------------------------------------------------HTPYEAYVSVDVRYEGKDFLSFVVYYYQFTGGAHGITFFETYNIDLKNSKVLKLYDIIKEEAEDTIKSNILKQIEQNNT--DFFPD-APMNILKDDIFSREFTISKDGLIIMYPHYDLAPYASGMPEFVIPWNVIEKFL----------------------

General information: TITO was launched using: RESULT: Template: 3CYG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 551 -61440 -111.51 -451.76 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain B : 0.64 3D Compatibility (PKB) : -111.51 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.467

(partial model without unconserved sides chains): PDB file : Tito_3CYG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3CYG-query.scw PDB file : Tito_Scwrl_3CYG.pdb: