Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIGALMLDIAGTELTQEDIELLQAPQVGGMILFARNIESPQQVRALTDHMRQV--RPDILIAVDQEGGRVQRLKSGFTLLPAMGRFGELYITQPQKALELAEQCGWLMATEVLAVGIDFSFAPVLDLNAISDVIGDRGFSKNIEDIAPLAGAFMRGMKKAGMANTGKHFPGHGSVKADSHVAAAIDSRSYDEIYNHDMQSFIKLMPE--LDALMPAHVIYDQVDLNPAGFSPFWIQEVLRNRLKFNGVLFSDDLSMQAACVAGGADARIQAALAAGCDMGLVCNDRSAACTALDGITNLELPNQERLERMRGRIPQIQVGETLSLGNDWQAVKTAIEEFKNSF 3GS6 Chain:A ((1-297)) -MGPLWLDVAGYELSAEDRAILAHPTVGGVILFGRNYHDNQQLLALNKAIRQAAARP-ILIGVDQEGGRVQRFREGFSRIPPAQ-----YYARAENGVELAEQGGWLMAAELIAHDVDLSFAPVLDMGFACKAIGNRAFGEDVQTVLKHSSAFLRGMKAVGMATTGKHFPGHGAVI---HLETPYDER---ETIAQDMAIFRAQIEAGVLDAMMPAHVVYPHYDAQPASGSSYWLKQVLREELGFKGIVFSDDLSM----VMGGPVERSHQALVAGCDMILICNKREAAVEVLDNLPIMEVPQAEAL------------------------------------

General information: TITO was launched using: RESULT: Template: 3GS6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1700 -55421 -32.60 -193.78 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : -32.60 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.455

(partial model without unconserved sides chains): PDB file : Tito_3GS6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3GS6-query.scw PDB file : Tito_Scwrl_3GS6.pdb: