Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKKGSFMPASTSQYYTRTAQILHWVMAIIFITAWVIGFYSGNFL-SYD-----------VDGSFKGDVITLHKNIATTIIFLVVIRLFWRYTHPAPQLPDTMSPTMKTLAHIGHLLLYVILVALPVTGCLFSWSAGHPA------PVLYLFEIPRLV-----------QDNPELLAIVKPLHIYISWFAGFLIVGHVLAALKHHFVDKDNVLNSMTKQPK 3L71 Chain:C ((33-203)) ---------------------NFGSLLAVCLMTQILTGLLLAMHYTADTSLAFSSVAHTCRNVQYGWLIRNLHANGASFFFICIFLHIGRGLYYGSYLY---------KETWNTGVILLLTLMATAFVGYVLPWGQMSFWGATVITNLFS--AIPYIGHTLVEWAWGGFSVDNPTLTRFFALHFLLPFAIAGITIIHLTFLHE------------------

General information: TITO was launched using: RESULT: Template: 3L71.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 378 -59259 -156.77 -417.32 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain C : 0.62 3D Compatibility (PKB) : -156.77 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.172

(partial model without unconserved sides chains): PDB file : Tito_3L71.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3L71-query.scw PDB file : Tito_Scwrl_3L71.pdb: