Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTHTAVANVKLNWTYFCLILASTFLQGSSFVATKILLSSMSPLWVATIR----FFIAALSLLPLIIYRYFKNPISLFNIPWLKLFVIGLFQTAGVMAFLNIGLGYTSPSTAAILMASNPLLVVILAMLILGERISIRALVGLIVAFIGVVICIGLGNTNSGGIGRGEVLVILASSCWAIATIINKKFNLHLDPWVITFW---QMLLGSLVLFLVALFSQ----------QPFTLPTTE-SMWLTFLWLAIPASTGAMGLWFAALKIGGAIHTSGFLFLCPLFSAIITYFVLGTVLTSQELIGGFLIGTGIYVLSRYR 5KO9 Chain:A ((2-271)) CGRTSCH-----------LPRDVLTRACAYQDRR--GQQRLPEWRDPDKYCPSYNKSPQSNSPVLLSRLHFEKDADSSERIIAPMRWGLVPSWFKESDPSKLQFNTTNCRSDTVMEKRSFKVPLG---------------------------------------KGRRCVVLADGFYEWQRCQGTN---QRQPYFIYFPQIEKVWDNWRLLTMAGIFDCWEPPEGGDVLYSYTIITVDSCKGLSDIHHRMPAILDGEEAVSKWLDFGEVSTQEALKLIHP--TENITFHAVSSVVNNSR-------NNTPECLAPVA

General information: TITO was launched using: RESULT: Template: 5KO9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1204 -100743 -83.67 -428.69 target 2D structure prediction score : 0.29 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -83.67 2D Compatibility (Sec. Struct. Predict.) : 0.29 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.138

(partial model without unconserved sides chains): PDB file : Tito_5KO9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5KO9-query.scw PDB file : Tito_Scwrl_5KO9.pdb: