Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYSLMSVTEKETSNSLYRQWQILSRLPTGKWIGTRELQEMLEREGIEISLRTIQRDLNQIAQRFPIESNKAVPQGWRWQSDAPIQSLPHMTSSQAVTFMMVEEHLKRLLPPSLLDEMGPWFDLAKRSLSTQNNVRQWINRVRIVPASQPLIPPVVDRAAQQAIYEGLLQDKQIECVYRARVNQGEDKTYILNPLALVQKGAVIYLICTRHDKTEVQTFALHRFKSAKVLESRALHPVNFDIDHYIDSGALGFRVDYNQPTESIQLTLTMTEQTAKTFYESQLSKDQTITPIEENIVEVTATVPFTSQLVWWLRSFGKKLLHIEPVQVHNAVREIEPDSK 3FHZ Chain:E ((16-64)) ------------ANRAGRQARIVAILSSAQVRSQNELAALLAAEGIEVTQATLSRDLEELG--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3FHZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 120 -17732 -147.76 -361.87 target 2D structure prediction score : 0.86 Monomeric hydrophicity matching model chain E : 0.55 3D Compatibility (PKB) : -147.76 2D Compatibility (Sec. Struct. Predict.) : 0.86 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.658

(partial model without unconserved sides chains): PDB file : Tito_3FHZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FHZ-query.scw PDB file : Tito_Scwrl_3FHZ.pdb: