Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MCNKASSDLTDYVIRQLGRTKNKRYE-AYVVSRIIHLLNDFTLKFVTQQFVRLSNKKIALTDLYFPQLGIHIEVDEGH---HFLRNSKMEYSL-NQIDEPLYSISQTESDAMREEDIISITGHKIFRVN------VF------KNQEGQPQNLENIHQQIDKIIEE--------------------------------IKTAKNKLIEAS--TFKEWNIETEYNPQTYIDLGRIS----------------LADNVVLKTTKDVCNCFGYSYKNYQRGGALH-------PYKKDTLIWFPRLYENKD----------WINTI-SPDGLTITEKSTDETITLKKLEEWKNGPQKRIVFARVKD--NLSSRAMYRFMG-------LYEFQKADLKDGAVWKRVKCEVQTYSPKETKC--------------------------- 2BMF Chain:A ((169-618)) MASIEDNPEIEDDIFRKKRLTIMDLHPGAGKTKRYLPAIVREAIKRGLRTLI-LAPTRVVAAEMEEALRGLPIRYQTGREIVDLMCHATFTMRLLSPIRVPNYNLIIMDEAHFTDPASIAARGYISTRVEMGEAAGIFMTATPPGSRDPFPQSNAPIMDEEREIPERSWNSGHEWVTDFKGKTVWFVPSIKAGNDIAACLRKNGKKVIQLSRKTFDSEYIKTRTNDWDFVVTTDISEMGANFKAERVIDPRRCMKPVILTDGEERVILAGPMPVTHSSAAQRRGRVGRNPKNENDQYIYMGEPLENDEDCAHWKEAKMLLDNINTPEGIIPSMFEPEREKVDAIDGEYRLRGEARKTFVDLMRRGDLPVWLAYRVAAEGINYADRRWCFDGVKNNQILEENVEVEIWTKEGERKKLKPRWLDARIYSDPLALKEFKEFAAGRK

General information: TITO was launched using: RESULT: Template: 2BMF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1624 105948 65.24 331.09 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 65.24 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.105

(partial model without unconserved sides chains): PDB file : Tito_2BMF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2BMF-query.scw PDB file : Tito_Scwrl_2BMF.pdb: