Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------MITQNKDILFRSPIMNKLMVYAYTGVVITMLFWGSAFNAMSYVIQYMPPLSAAAERFTIASL-------GLFILFAAIGKLSWAALRQNLVIYLIIGIIGIAGFNLGCFYGLQTTSAVNGALIMATTPLITLLLTILLDGEKLTLNKFIGVVF---------------------------------------GLSGVLFVISHGHI--------STLLHLKIAIGDLFILLGGVSFCLANVLSRRYVKNATPLETTTFSM------MFGALTLIILSVIFEHPSQAIAMAPITAHLAMGYVI------ICSTMIAYLFWFNGIQKLGAGRASIFFNFVPVFSMLVALLAGQSLNIWQ-----LIGTALVMLGVMSSGGFIQIKRPALIAKPCTK-- 2G0D Chain:A ((1-409)) KKNIKRNVEKIIAQWDERTRKDFGELTLSTGLPGIILMLAELKNKDNSKIYQKKIDNYIEYIVSKLSTYGLLTGSLYSGAAGIALSILHLREDDEKYKNLLDSLNRYIEYFVREKIEGFNLENITPPDYDVIEGLSGILSYLLLINDEQYDDLKILIINFLSNLTKENNGLISLYIKSENQMSQSESEMYPLGCLNMGLAHGLAGVGCILAYAHIKGYSNEASLSALQKIIFIYEKFELERKKQFLWKDGLVADELKKEKVIREASFIRDAWCYGGPGISLLYLYGGLALDNDYFVDKAEKILESAMQRKLGIDSYMICHGYSGLIEICSLFKRLL-NTKKFDSYMEEFNVNSEQILEEYGDESGTGFLEGISGCILVLSKFEYSINFTYWRQALLLFDDFLKGG

General information: TITO was launched using: RESULT: Template: 2G0D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1528 -87564 -57.31 -271.94 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -57.31 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.230

(partial model without unconserved sides chains): PDB file : Tito_2G0D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2G0D-query.scw PDB file : Tito_Scwrl_2G0D.pdb: