Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTHTALDQLKTLTTVVADSSDLEAIRKFRPLDATTNPSLITAAAEQPESKELIEDAYYQAKEEG--YKNDELI-ERTIDILTVKFGVEILKLIEGRVSTEVDTALSYDTEATIQKAHELCELYKGYGIDQSRILIKIASTWEGIQAAKVLEAEGIACNLTLLFGLHQAQACADAKVTLISPFVGRILDWYKKAEGVDSYP-IEKDPGVVSVKKIYTYYKQQNIPTQVMGASFRSIDQVLGLAGCDLLTISPSLLTQLEQDTRTVDAALDANKAKQAEAIVR-PAQDEQSFKDELNHDLMAFQLLQGGVDGFIKARDQLSLLLRQSFGIDAEIKS 3TK7 Chain:A ((26-345)) -QKSVLEQLKQVTMVVADTGDFELIKKYKPVDATTNPSLILKAVKEQKYSNLVAETISKVKANNPDLNSDDLVKEIAIEIL-VSFGIKILDVIEGKVSSEVDARVSFNSATTIDYAKRIIARYESNGIPKDRVLIKIAATWEGIKAAKLLQKEGINCNLTLIFDKAQAKACAEAGVYLVSPFVGRITDWQMQQNNLKTFPAIADDDGVNSVKAIYKLYKSHGFKTIVMGASFRNVEQVIALAGCDALTISPVLLEEL----KNRDEHLEVKLTKNDDVVTQSPQISEADFRWLMNENAMATHKLAEGIRLFTKDTIELENIIKQNL--------

General information: TITO was launched using: RESULT: Template: 3TK7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1745 -5678 -3.25 -18.03 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -3.25 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.531

(partial model without unconserved sides chains): PDB file : Tito_3TK7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TK7-query.scw PDB file : Tito_Scwrl_3TK7.pdb: