Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFKSFFPSPRYFFISVVIWLALNMVLWYTGGDHWGQYLGFPQGYADAELPIGVSRFWSPAFLWFYLWFLVSTALFAGFWKIISNNPWQRWSIWGAAFILFNIWFAVQVNVAINAWYVPFWDLIQKMLSSGGGDISTLYSETLVFLYIAMVYVTLGVITAFF----TSHYVFRWRTAMNEYYTEH---WEKLRHIEGASQRVQEDTMRFATIMEDLGVELVKSVITLIAFLPILFHL-SKYVLVLPLVGELNHSLVWAAIVWSIFGTVLLMVVGIKLPGLQFNNQKVEAAYRKELVYGEDHAE--RA--QPVTLKELFANVRKNYFRLYFH----YAYFNMTATWYGQLDILYNLVVLFPSIAAGKLTLGLMQQIMNVFGRVRDSFQYLISSWKTIIELLSIYKRLKAFESILHK 5EG1 Chain:B ((29-326)) --------------------------------------------------------------------------------------------------LFITSLSSIIISISPLILAKITDLLSGSLS--NFSYEYLVLLACLYMFCVISNKASVFLFMILQSSLRINMQKKMSLKYLRELYNENITNLSKNNAGYTTQSLNQASNDIYILVRNVSQNILSPVIQLISTIVVVLSTKDWFSA-------------GVFFLYILVFVIFNTRLTGSLASLRKHSMDITLNSYSLLSDTVDNMIAAKKNNALRLISERYEDALTQENNAQKKYWLLSSKVLLLNSLLAVILFGSVFIYNILGVLNGVVSIGHFIMITSYIILLSTPVENIGALLSEIRQSMSSLAGFIQRHAE---

General information: TITO was launched using: RESULT: Template: 5EG1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 855 -84058 -98.31 -298.08 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.56 3D Compatibility (PKB) : -98.31 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.281

(partial model without unconserved sides chains): PDB file : Tito_5EG1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EG1-query.scw PDB file : Tito_Scwrl_5EG1.pdb: