Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFMTESRLTHLKQLEAESIHIIREVAAEFENPVMLYSIGKDSAVMLHLALKAFYPAKLPFPLLHVDTGWKF-KDMIAFRDNMAKTHGFDLIVHQNKEGREA---GINPFDHGSSKYTDIMKTQALKQALDKYQFDAAFGGARRDEEKSRAKERVYSFRDSKHRWDPKNQRPELWNLYNGKVNKGESIRVFPLSNWTELDIWQYIYLENIQIVPLYFSAVRPVVERSGTLIMVDDERMRLKEGEVPQMKSVRFRTLGCYPLTGAVESEADTLPEIIQEMLLATSSERQGRMIDHDEAGSMEKKKQEGYF 5TW7 Chain:C ((208-417)) ----------MPNYIEEAVAKIREQVGS-DEVILGLSGGVDSSVAAALIHRAIG---DQLTCVFVDHGLLRLNEGKMVMDMFARNLGVKVIHVDAEGQFMAKLAGVTDPEKKRKIIGAEFIEVFDAEEKKLTNAKWLAQGTIYPDVIE------------------------------------KLKLLEPLRDLFKDEVRELGVALGLPREMVYRH---------------------------------PFPGPGLGVRILGE--------------------------------------------

General information: TITO was launched using: RESULT: Template: 5TW7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 819 -51695 -63.12 -292.06 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain C : 0.60 3D Compatibility (PKB) : -63.12 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.407

(partial model without unconserved sides chains): PDB file : Tito_5TW7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5TW7-query.scw PDB file : Tito_Scwrl_5TW7.pdb: