Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------MAFPHHTEHHAIQRSGWLRASVLGANDGIISVTSLIMGMAASGASSHTLFITCVAGLISGATSMAAGEYISVKSQEDIEKSDLAIEAKELKKYPQKELDELTQIYISRGLSKELAK-EVAIQLTTHDALGAHARDEIGIHENTAANPIQAALSSAASFSFGAFFPMLAILFSPEHLIMPSVLITGIAALAILGALSSYFAGTSKIKGSLRITLWGILAMAFSSWIGSLFNVTPL- 4EGD Chain:A ((27-253)) GRNAQIKDTFNQTLKLYPTKNLDDFYDKEGFRDQEFKKGDKGTWIVNSEMVIEP----KGKDMETRGMVLYINRNTRTTKGYYFISEMTPKDDEKRYPVKMEHNKIIPTKPLP-------NDKLKKEIENFKFFVQYGNFKDINDYKDGDISYNPNVPSYSAKYQLNND-----DYNVQQLRKRYDIPTKQAPKLLLKGDGDLK----------GSSVGSRSLEFTFVE--NKEENIYFTDSVQYTPSE

General information: TITO was launched using: RESULT: Template: 4EGD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 880 51103 58.07 249.28 target 2D structure prediction score : 0.37 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 58.07 2D Compatibility (Sec. Struct. Predict.) : 0.37 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.245

(partial model without unconserved sides chains): PDB file : Tito_4EGD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EGD-query.scw PDB file : Tito_Scwrl_4EGD.pdb: