Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPFFGFTPSEGLLNDIQTGIANKNSSEPLYPLRDKIALQLNEEIIENVL---TQLVQHFPASEKRDTAEKLAGYV-KSTVAVLLKQLLSKAPNDVVKQSVEFSEKSLFKDPQGQYKVGVAL-DAGLVTNLKHNFAELQAGNDINKAALAELYKQFGDAMV-RHFMSDFNKTLDLGMIKRKAADIGAAAVTKAVHIAIDKLIPSLNRTELKAMAEYHDGLFFN 1KN0 Chain:A ((25-208)) --CFG---------------QCQYTAEEYQAIQKALRQRLGPEYISSRMAGGGQKVCYIEGHRVINLANEMFGYNGWAHSITQQNVDFVDLNNGKFYVGVCAFVRVQLKDGSYHEDVGYGVSEGLKSKALSLEKARKEAVTDGLKRALRSFGNALGNCILDKDYLRSLNK-----LPRQLPLEVDLTKAKRQ------------DLEPSVEEARYNSC----

General information: TITO was launched using: RESULT: Template: 1KN0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 661 9992 15.12 56.13 target 2D structure prediction score : 0.34 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 15.12 2D Compatibility (Sec. Struct. Predict.) : 0.34 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.204

(partial model without unconserved sides chains): PDB file : Tito_1KN0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1KN0-query.scw PDB file : Tito_Scwrl_1KN0.pdb: