Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPITSENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEK-EVKIDISVNSATTNSNVMVEYGKNEIEKDMSLIFDLVDYVSY-PQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQTEKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLT--LHEVKPDQINARNVILANVQNDQKTYINQVVIETKITKKGHSEVL-YQE--EKEGLQIAPNTNFSFPTALNGQPLTPGEYHLTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNNWIYILVASLFLLFILLCILLFVLRKKEEKEK 3GFU Chain:A ((16-195)) -----------------------------------------------------------------DALTITVTNNDKERTFGGQ----AWVDNIVEKD----------------TRPTFVVTPSFFKVKPNGQQTLRIIMASDHLPKDKESVYWLNLQDIPPAL-------EGSGIAVALRTKLKLFYRPKA--LLEGRKGAEEGISLQSRP-DG--RTMLVNTTPYIFA-IG-SLLDGN---GKKIATDNGTTQKLLMFMPGDEVQVK---------GNVVKVDSLND-YG--------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3GFU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 711 30811 43.33 178.10 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : 43.33 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.269

(partial model without unconserved sides chains): PDB file : Tito_3GFU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3GFU-query.scw PDB file : Tito_Scwrl_3GFU.pdb: