Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSSDHAHYWS--VFFFVLILYAYHHGIFRSSTSLQAFIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAIGFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVAGLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL 4ER8 Chain:A ((1-165)) MSEYRRYYIKGGTWFFTVNLRN------RRSQLLTTQYQMLRHAIIKVKRDRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGL---------------KNIWQPRFWEHAIRNTKDYRHHVDYIYINPVKHG-WVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDFSAGERIIS-----

General information: TITO was launched using: RESULT: Template: 4ER8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 710 -55456 -78.11 -340.22 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -78.11 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.266

(partial model without unconserved sides chains): PDB file : Tito_4ER8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ER8-query.scw PDB file : Tito_Scwrl_4ER8.pdb: