Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MNKMSEKYQVTKKDLRKANYRWLMSVC-TFNYQTQQGASVTYALS----PILRKLYKDDDEYIEALNNHFQYYNTQPW-------------LAAIILGACVAMEEKQGLEAKDAINDFKVGTMGPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYKQGMKIVTEYGEKINYLTE--AASVLGLTVVGSLI--------ASVISVSTPLKFTFGE-VSMKIQPMLDKI-----LPTLIPVIIT--AAAYYLLSKKKISMTILIL--------LVIVFAMVMAAFGILA------- 5CZL Chain:A ((23-337)) LPRAWADTAWESYKSRFMMADGRIVDTGNGSVSHTEGQGFAMLLAVAKNDRPAFDKLWQWTDKTLRNKDNGLFYWRYNPVAPDPIADKNDATDGDTLIAWALLRAQQQWGDKSYGIASDAITASLLKSTVITFAGHQVMLPGAKGF------NRNDHVNLNPSYFIFPAWQAFAARTHLTAWRKLQSDGKALLGKMAWGKAQLPSDWVALRADGRMEPAKEWPPRMSYDAIRIPLYLSWADPQSALLTPWKSWFQSYPRLQTPAWVNVNTNDVAPWFMTGGLLAVRDLTTGEAQDDPQLSAQDDYYSASLKMLVWLAKNDR

General information: TITO was launched using: RESULT: Template: 5CZL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1330 -54435 -40.93 -207.77 target 2D structure prediction score : 0.40 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -40.93 2D Compatibility (Sec. Struct. Predict.) : 0.40 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.098

(partial model without unconserved sides chains): PDB file : Tito_5CZL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5CZL-query.scw PDB file : Tito_Scwrl_5CZL.pdb: