Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------MLKNFKEVIAA---------------ILPMTVLIVILTFVFAPLEGEDLASFLVGAA--IMMIGM----TLFLFGADYSMMEVGDLVGKYM-----IKKKSLTV-----LVSLGFAIGIVITIAEPSVQVLGQQ------VYDISDGEIGRVLLIGI---VSVGTGVFLAFALLRVVFKLS----YYQLMAIG----------YIGVLIA------------SFFTSSEFMPIAFDSGG---------------VTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGI---ASLGPILA----VMILGVIFQ----------- 5TF2 Chain:A ((26-404)) ELYRAPFPLYALQVDPSTGLLIAAGGGGAAKTGIKNGVHFLQLELINRLSASLLHSHDTETRATMNLALAGDILAAGQDAHCQLLRFQAHLE-LRVENLQAVQTDFSSDPLQKVVCFNHDNTLLATGGTDGYVRVWKVPSLEKVLEFKAHEGEIEDLALGPDGKLVTVGRDLKASVWQKDQLVTQLHWQEPTFSSTPYRYQACRFGQVPDQPAGLRLFTVQIPHKRLRQPPPCYLTAWDGSNFLPLRTKSCGHEVVSCLDVSESGTFLGLGTVTGSVAIYIAFSLQCLYYVREAHGIVVTDVAFLPEKGRGPELLGSHETALFSVAVDSRCQLHLLPSR

General information: TITO was launched using: RESULT: Template: 5TF2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1192 -116192 -97.48 -514.12 target 2D structure prediction score : 0.27 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -97.48 2D Compatibility (Sec. Struct. Predict.) : 0.27 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.027

(partial model without unconserved sides chains): PDB file : Tito_5TF2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5TF2-query.scw PDB file : Tito_Scwrl_5TF2.pdb: