Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVHPMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVEAAQQKLKAAT--TREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARYTEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAMIVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPRKDVTESAKPADNKQPKNSTNNKGRNAGKQQKRK 3WO7 Chain:B ((9-240)) ---------------------------------SEGIWNHFFVYPMSWLITTVANLLNGSYGLSIIIVTILIRLALLPLTLKQQKSM----RAMQVIRPEMEAIQKKYKEKASKDPKVQQEMQKELLGLYQKHGVNPMA--GCLPLFIQLPILMAFYFAIMRTEEIRYHTFLWFDLGQPDYILPFVAGITTYFQFKMTMS------QMQMKVMLYVMPVMIIIAGLSLPSALSLYWVIGNIFMIIQTYFIVVKAPP-------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3WO7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 693 -116879 -168.66 -559.23 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : -168.66 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.316

(partial model without unconserved sides chains): PDB file : Tito_3WO7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3WO7-query.scw PDB file : Tito_Scwrl_3WO7.pdb: