Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACETSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSPDIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRLADLRKSNELTYLRPDAKSQVTVEY-DDQGQ--PERVDTIVISTQHDDAVDNETIRHDVIEKVVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVPEEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALKASLAK 1O93 Chain:A ((20-394)) -----MFTSESVGEGHPDKICDQISDAVLDAHLKQDPNAKVACETVCKTGMVLLCGEITSMAMIDYQRVVRDTIKHIGYDDSAKGFDFKTCNVLVALEQQSP--------------ED-------VGAGDQGLMFGYATDETEECMPLTIVLAHKLNTRMADLRRSGVLPWLRPDSKTQVTVQYVQDNGAVIPVRVHTIVISVQHNEDITLEAMREALKEQVIKAVVPAKYLDEDTIYHLQPSGRFVIGGPQGDAGVTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAEPLSISIFTYGTSKKTERELLEVVNKNFDLRPGVIVRDLDLKKPIYQKTACYGHFGRS--EFPWEVPKKL---------

General information: TITO was launched using: RESULT: Template: 1O93.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2213 -31793 -14.37 -88.31 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.88 3D Compatibility (PKB) : -14.37 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.528

(partial model without unconserved sides chains): PDB file : Tito_1O93.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1O93-query.scw PDB file : Tito_Scwrl_1O93.pdb: