Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVKQATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDD-KSPRGTRIFGPVARELRENNFMKIVSLAPEVL 5GAD Chain:L ((1-123)) MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIKEAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGPVTRELRSEKFMKIISLAPEVL

General information: TITO was launched using: RESULT: Template: 5GAD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain L - contact count / total energy / energy per contact / energy per residue : 635 -63199 -99.53 -518.02 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain L : 0.91 3D Compatibility (PKB) : -99.53 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.91 QMean score : 0.561

(partial model without unconserved sides chains): PDB file : Tito_5GAD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5GAD-query.scw PDB file : Tito_Scwrl_5GAD.pdb: