Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALTLGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFFAVTIGVNFVLLLFNWTKTLNVDMWNVWGKAL---TAYLVYYVSGSLAAGFLTAMVQVILELKLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIM---DKIPALNKRADTNALKKKIGIF--SENSVMGFIIGLGLGFAAAYGVSGSLNLAIQT-ATALSLFPMISKMFMQSLSPLADA---------MSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLV------PVFIGYAIILPGNQVLPLAGIINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKKGQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAELQK 1TUB Chain:A ((1-440)) ---MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQ---MPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGK-EDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFSVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRG-HFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCD-----PRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYEPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLM--YAKRAFVHWYVGEGME----------------------EGEFSEAREDMAALEKDYEEVGVDSV

General information: TITO was launched using: RESULT: Template: 1TUB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2682 -184821 -68.91 -444.28 target 2D structure prediction score : 0.38 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -68.91 2D Compatibility (Sec. Struct. Predict.) : 0.38 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.193

(partial model without unconserved sides chains): PDB file : Tito_1TUB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TUB-query.scw PDB file : Tito_Scwrl_1TUB.pdb: