Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGILLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITSDRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVSLNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHY-LEDIRLRLSAEIG-RGLTLLEAKGGYNR---KSKEVLLIVVTDHEFSRMKPLIEE-ID----PSAFVIVSSVAEVMGRGFTYHPIE 1VFJ Chain:B ((4-107)) -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------IVAIVRPEKLNEVLKALFQ-AEVRGLTLSRVQGHG--MELHEKVRLEIGVSEPFVKPTVEAILKAARTGEVGDGKIFVLPVEKVYRIRTGEE---

General information: TITO was launched using: RESULT: Template: 1VFJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 290 -9200 -31.72 -116.46 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.43 3D Compatibility (PKB) : -31.72 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.43 QMean score : 0.439

(partial model without unconserved sides chains): PDB file : Tito_1VFJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VFJ-query.scw PDB file : Tito_Scwrl_1VFJ.pdb: