Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREMPFNHGYAVFAGLERLVN-YLENLTFNDSDIAYLRGL-------EVYPEGFLEYLQNFE---FKATVRSAREGELVFANEPLIQVEGP----LAHCQLVETALLNMVNFQTLIATKAARIKSVIG-------------EDPLLEFGTRRAQELDAAVWGTRAAYIGGADATSNVRAG----KIFGIP--A-S---GTHAHSLVQSYG--NDYEAFMAYAKTH--KDCVFLVDTYDTLKSGVPSAIRV-AKE-LGDK--INFQGVRIDSGDMAYISKRVREQLD--------AAGFT----EAKIYASNDLDEATILNLK----MQKAKI--DVWGVGTKLITAYDQPALGAVFKLVSIEDDEGKMMDTIKLS-SNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEEAIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEEYKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA 4LVF Chain:A ((8-486)) ------EFNILLATDSYKVTHYKQYPP----NTSKVYSYFECREK-KYEETVFYGLQYILNKYLKGKVVTKEKIQEAKDVYKEHFQDDVFNEKGWNYILEKYDGHLPIEIKAVPEGFVIPRGNVLFTVENTDPECYWLTNWIETILVQ-SWYPITVATNSREQKKILAKYLLETSGNLDGLEYKLHDFGYRGVSSQETAGIGA-SAHLVNFKGTDTVAGLALIKKYYGTKDPVPGYSVPAAEHSTITAWGKDHEKDAFEHIVTQFSSVPVSVVSDSYDIYN-ACEKIWGEDLRHLIVSRSTQAPLIIRPDSGNPLDTVLKVLEILGKKFPVTENSKGYKLLPPYLRVIQGDGVDINTLQEIVEGMKQKMWSIENIAFGSGGGLLQKLTRDLLNCSFKCSYVVT-NGLGINVFKDPVADPNKRSKKGRLSLHR-TPA------GNFVTLEEGKGD------------------LE-EYGQDLLHTVFKNGKVTKS-YSFDEIRKNAQLNIEL--------------------------------------------

General information: TITO was launched using: RESULT: Template: 4LVF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2093 56013 26.76 138.64 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 26.76 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.423

(partial model without unconserved sides chains): PDB file : Tito_4LVF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LVF-query.scw PDB file : Tito_Scwrl_4LVF.pdb: