Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHENSDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNKNFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK 3N39 Chain:D ((60-147)) ---------------------------------------------------EPYILIVPSYGGGGTA-----GAVPRQVIR-FLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCGVPWLYRFELMGTQSDIENVRKGVTE---

General information: TITO was launched using: RESULT: Template: 3N39.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 320 -48574 -151.79 -551.97 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain D : 0.74 3D Compatibility (PKB) : -151.79 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.456

(partial model without unconserved sides chains): PDB file : Tito_3N39.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3N39-query.scw PDB file : Tito_Scwrl_3N39.pdb: