Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQILGGNNMAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIELS--KKGY-YDGVIFHRVIPDFMIQGGDPTG-TGMGGESIYGESFEDEFSRELFNLR----GALSMANSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHVLEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE 3R49 Chain:A ((19-143)) ------------------------------GRVVLELKADVVPKTAENFRALCTGEKGFGYKGSTFHRVIPSFMCQAGDFTNHNGTGGKSIYGSRFPDE----NFTLKHVGPGVLSMANAGPNTNGSQFFICTIK---------------------------TDWLDGKHVVFGHVIEGMDVVKKI------------------------

General information: TITO was launched using: RESULT: Template: 3R49.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 563 24967 44.35 213.39 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : 44.35 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.548

(partial model without unconserved sides chains): PDB file : Tito_3R49.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3R49-query.scw PDB file : Tito_Scwrl_3R49.pdb: