Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKAITDATFEQET-KDGLVLVDFWATWCGPCRMQGPILDKLSEELSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTVEQIKAIIAELS 2YZU Chain:A ((4-105)) -PIEVTDQNFDETLGQHPLVLVDFWAEWCAPCRMIAPILEEIAKEYEG-KLLVAKLDVDENPKTAMRYRVMSIPTVILFKDGQPVEVLVGAQPKRNYQAKIEKH-

General information: TITO was launched using: RESULT: Template: 2YZU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 434 2841 6.54 28.12 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : 6.54 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.673

(partial model without unconserved sides chains): PDB file : Tito_2YZU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2YZU-query.scw PDB file : Tito_Scwrl_2YZU.pdb: