Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTMPKRQRSDEKAAHHVQLSVESGSRFDAYIRGWMQGTVEQPLLRPSLLPPLTPLPTLAAPSKSGAAQTPATASAATTVGDLSRLCEWTPPSAVMPASRTAVVYDTSMLD-HVSPDAGDYERPARLQSTLDHLTVIGLLPCCQRIPARTAKKHELRRVHCRELIDTVDQLDFFMGIQEGRGSVIGQDLFASEHTSRAARMAAGCVIEAVKAVLSGAATNAFAIVRPPGHHAGPANAAGYCLYNNVAVAARAAQAELAAAQAEHNPAGDAQQPRILILDWDVHHCDGTESIFYDDPSVLVISLHQY-------------------GNGHG-----------------------------------------------------------------HVLRKV----------------SSAAAKNPAEIS-------------TPSKEAI-----TADDL------AALLSGGAI-EPPPVSAPEA------------------------------------SRPEAASDPPQGSTEGRRVRAAV------------DYNSLAAQIEEEGDAEIAALF----GVDLNAASTSSSNSSSASTSSSVSADSTSVARNGT---RPV--HYAGDT-VGVSFDETPRQRATGAEEEE------LFY-------PGTGH------------LNRVGGDATP--------------AAQGRNINIPWPTHGMGDLEYLQLLHEVVLPAAREFRPELVLISCGFDSASGDLLGSMCLTPSGYYIMT-RLMAQNFPKLVVALEGGYNVRNVALCSEAVMRALLESSGCPGDRLPKSRMLWCQASSLVADIKRMHAPYWSCFSPNM-------- 5G0J Chain:A ((17-772)) --------------------VQGKSK----------------------------------------------------------------------ATGTGLVYVDAFTRFHCLWDASHPECPARVSTVMEMLETEGLLGRCVQVEARAVTEDELLLVHTKEYVELMKSTQNMTEEELKTLAEKYDSVYLHPGFFSSACLSVGSVLQLVDKVMTSQLRNGFSINRPPGHHAQADKMNGFCMFNNLAIAARYAQKRH-------------RVQRVLIVDWDVHHGQGIQYIFEEDPSVLYFSVHRYEDGSFWPHLKESDSSSVGSGAGQGYNINLPWNKVGMESGDYITAFQQLLLPVAYEFQPQLVLVAAGFDAVIGDPKGGMQVSPECFSILTHMLKGVAQGRLVLALEGGYNLQSTAEGVCASMRSLLGDPCPHLPSSGAPCESALKSISKTISDLYPFWKSLQTFEGGPLSEVSPLPAPVCAEVKVSSPITGLVYDQRMMLHHNMWDSHHPELPQRISRIFSRHEELRLLSRCHRIPARLATEEELALCHSSKHISIIKSSEHMKPRDLNRLGDEYNSIFISNESYTCALLAAGSCFNSAQAILTGQVRNAVAIVRPPGHHAEKDTACGFCFFNTAALTARYAQSITRESLRVLIVDWDVHHGNGTQHIFEEDDSVLYISLHRYEDGAFFPNSEDANYDKVGLGKGRGYNVNIPWNGGKMGDPEYMAAFHHLVMPIAREFAPELVLVSAGFDAARGDPLGGFQVTPEGYAHLTHQLMSLAAGRVLIILEGGYNLTSISESMSMCTSMLLGDSPPSLDHLTP---LKTSATVSINNVLRAHAPFWSSLRVNIPESLRLSL

General information: TITO was launched using: RESULT: Template: 5G0J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2907 -44287 -15.23 -84.52 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -15.23 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.349

(partial model without unconserved sides chains): PDB file : Tito_5G0J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5G0J-query.scw PDB file : Tito_Scwrl_5G0J.pdb: