Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNQEVKSGKVLSPSTPWTQRPVPGIEVADEQQTLKATFTEPTIECPECHALVTRTAISFNAYVCPQCDEHLRMKARDRLNWFFDNV-VAELGQEFSAKDPLKFVDSKPYPDRMREAQTKTGETEALIAMQGNLNGVDMIACAFEFDFMAGSMGTVVGDRFVKAAELAIEKRQPLICFAASGGARMQEGMLSLMQMARTSAAIQKLKDTGLPYIVVLTHPVYGGVTASLAMLGDIHIAEPK-A-MIGFAGKRVIEQTVRETLE-EPFQRAEYLL-DHGVVDQIVH-RHALRDTVSRLVSKLMNLP 1X0U Chain:E ((36-256)) -------------------------------------------------------------------HSKGKLTARERLALLFDDGKFNEIMTFATT-RATEFGL---D--------KQRFYGDGVVTGWGKVDGRTVFAYAQDFTVLGGSLGETHANKIVRAYELALKVGAPVVGINDSGGARIQEGALSLEGYGAVFKMN-VMASGVIPQITIMAGPAAGGAVYS-PALTDFIIMIKGDAYYMFVTGPEITKVVLGEEVSFQDLGGAVVHATKSGVVHFMVDSEQEAINLTKRLLSYLPS--

General information: TITO was launched using: RESULT: Template: 1X0U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 1219 -53664 -44.02 -249.60 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain E : 0.69 3D Compatibility (PKB) : -44.02 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.469

(partial model without unconserved sides chains): PDB file : Tito_1X0U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1X0U-query.scw PDB file : Tito_Scwrl_1X0U.pdb: