Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQH 2CRH Chain:A ((28-102)) WYAGPMERAGAESILANRSDGTFLVRQRVKDAAEFAISIKYN-----------------VEVKHIKIMTAEGLYRITE---KKAFRGLTELVEFY

General information: TITO was launched using: RESULT: Template: 2CRH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 282 -42501 -150.71 -566.67 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -150.71 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.470

(partial model without unconserved sides chains): PDB file : Tito_2CRH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2CRH-query.scw PDB file : Tito_Scwrl_2CRH.pdb: