Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WYWGPITRWEAEGKLANVPDGSFLVRDSSD-DRYLLSLSFRSHGKTLHTRIEHSNGRFSFYEQPDVEGHTSIVDLIEH 2IUG Chain:A ((12-86)) WYWGDISREEVNEKLRDTADGTFLVRDASTKMHGDYTLTLRKGGNNKLIKIFHRDGKYGFSD---PLTFSSVVELINH

General information: TITO was launched using: RESULT: Template: 2IUG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 263 -8128 -30.90 -109.83 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -30.90 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.612

(partial model without unconserved sides chains): PDB file : Tito_2IUG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2IUG-query.scw PDB file : Tito_Scwrl_2IUG.pdb: