Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKAITDATFEQET-KDGLVLVDFWATWCGPCRMQGPILDKLSEELSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTVEQIKAIIAELS 1NSW Chain:C ((2-103)) -TMTLTDANFQQAIQGDGPVLVDFWAAWCGPCRMMAPVLEEFAEAHAD-KVTVAKLNVDENPETTSQFGIMSIPTLILFKGGRPVKQLIGYQPKEQLEAQLADV-

General information: TITO was launched using: RESULT: Template: 1NSW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 438 -4840 -11.05 -47.92 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain C : 0.86 3D Compatibility (PKB) : -11.05 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.521

(partial model without unconserved sides chains): PDB file : Tito_1NSW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1NSW-query.scw PDB file : Tito_Scwrl_1NSW.pdb: