Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------DVPKHGQV--DSRHGQGSSNHGNNKPDKQFL-----------------WGWRCQVAL------IEHVDGKKVEVGKVVTEIGGEGSLHNVQLGE----KGYVRFKTRQNCYPD-IIEGTM---DGDRFELGHKRRWTVVRTTKVPGEDKAPLVAETQ 5MMI Chain:S ((68-237)) LKAEFEEFQRPPNQKPQLSDVLPDDFQAPEPGTPEYNDIINQFLPKKGPPPPREEIFAVVVIGSRQYIVIPGRWIYTQRLKGATVNDKIVLNKVLLVGTKASTYIGTPIVTNAAVHAVVEEQLLDDKVIVFKYKKKKNYRRNIGHRQPITRIKITGITGYEDYPASTLEA

General information: TITO was launched using: RESULT: Template: 5MMI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain S - contact count / total energy / energy per contact / energy per residue : 276 11523 41.75 92.92 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain S : 0.66 3D Compatibility (PKB) : 41.75 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.282

(partial model without unconserved sides chains): PDB file : Tito_5MMI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5MMI-query.scw PDB file : Tito_Scwrl_5MMI.pdb: