Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEQCYTDVTEFAI-----PASTQKLYLSPVLDGFNSEIIAYNLSTSPNLEQVQTMLEQAFTEKHYENT--ILHSDQGWQYQHDSYH-----------------QFLEGKGIQAS----MSRKGNSQDNGMMESFFGILK-SEMFYGYEK-----------TFKSLNQLEQAIVDYIDYYNNKRIKVKLK----GLSPVQYRTKSFG 1BCO Chain:A ((263-471)) MQWINGDGYLHNVFVRWFNGDVIRPKTWFWQDVKTRKILGWRCDVSENIDSIRLSFMDVVTRY-GIPEDFHITIDNTRGAANKWLTGGAPNRYRFKVKEDDPKGLFLLMGAKMHWTSVVAGKGWGQAKP-VERAFGVGGLEEYVDKHPALAGAYTGPYGDRAVDAELFLKTLAEGVAMFNA-RTGRETEMCGGKLSFDDVFERE--

General information: TITO was launched using: RESULT: Template: 1BCO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 636 5235 8.23 33.34 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 8.23 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.358

(partial model without unconserved sides chains): PDB file : Tito_1BCO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1BCO-query.scw PDB file : Tito_Scwrl_1BCO.pdb: