Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLFFVFSERRSISWHESVKLSLGVLAICVMSLAKLINELNAQQKKAATTMAQNCLVLAGAGCGKTKTIVARAAYLV-DQGLPANQIQILTFTRRAASEIVARVEQHVGAQAKGLRASTFHTFCMYLLRRNPQAFGLTQ-FSIIDRDDQL-LMFRLLRGKDKDNVLPKAAELCDLYSYARNTQSKL--SEALLKQLPEAYEYKSQIAELMKTYEQRKRERNFLDYDD-ILSIVDVHLQNSEVLTNWVAGFCQALLVDEMQDTNPLQWALLQPLIGKVKLFC-VGDDAQSIYGFRGADFENIHSFKERIPDAVIHTLDLNYRSTQEILDLSNWLLAHSPIDYQKQLQAHRGKGQKPQLHLFG-NEFEEANWIAQDLIERHQQGANWY-DHMVLVRSGYSARYLEGAFIAAEIPYRFIGGVKLLESAHVKDVLSLLRIVSNPQDDLAWMRFLTLWD-GIGDVGASKLAQELMSICEVEERCQRLERHGKVPLQAILILKQLDVLQQHVEASIGLALDALSEQLEQNYKTKDWSRRVRDFDLVKQLARKHASLGEFLEEYVLEPISISEIEKAGDDDLVTLITIHSAKGAEQKVCYVPHVSPNQYPYARAQGDFDEVEEERRVLYVALTRAENELILTKQNLNTWSQDTYDEQGRKIESYFLNDLPAHLVQAKIHRQVPQPFAKKQWNTSRTVNLGFGIDLD 2PJR Chain:F ((7-432)) ---------------------------------QLLAHLNKEQQEAVRTTEGPLLIMAGAGSGKTRVLTHRIAYLMAEKHVAPWNILAITFTNKAAREMRERVQSLLGGAAEDVWISTFHSMCVRILRRDIDRIGINRNFSILDPTDQLSVMKTILKEK---NIDPKKFEPRTILGTISAAKNELLPPEQFAKRASTYYE--KVVSDVYQEYQQRLLRNHSLDFDDLIMTTIQLFDRVPDVLHYYQYKF-QYIHIDEYQDTNRAQYTLVKKLAERFQNICAVGDADQSIYRWRGADIQNILSFERDYPNAKVILLEQNYRSTKRILQAANEVIEHNVNRKPKRIWTENPEG-KPILYYEAMNEADEAQFVAGRIREAVERGERRYRDFAVLYRTNAQSRVMEEMLLKANIPYQIVGGLKFYDRKEIKDILAYLRVIANPDDDLSLLRIINVPKRGIGASTIDKLVR----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2PJR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 1894 3615 1.91 8.69 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain F : 0.70 3D Compatibility (PKB) : 1.91 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.376

(partial model without unconserved sides chains): PDB file : Tito_2PJR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2PJR-query.scw PDB file : Tito_Scwrl_2PJR.pdb: