Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDRLQQFYIFTEVAKRQSFSEVANQLDLPRSTVTSAIQQLETHYGVRLFHRTTRKVSLTQDGQRILPECQNLLFDYEQLEQLIQTQKQHYRGTLKISMPSR-I-VHQVIIPELPDFYHRYPDIHLQLNSSDDLTDLIEKGIDCVVRVGELDNSSLIARFIGHLVMVNCASTQYLQDYGIPEQLNDLEQHKLINYAGAVGEKQGEFIYQGG-TVMMDSALSVNNTEAYIAAACAGLGIIQLPYYDVQDKIEQGILVEVLSAY-IAPSLPLNILYPNRSYIPKRLEVFMNWVGEVLNRKCIVIF 3HHF Chain:A ((5-207)) -----------------------------------------------------------------------------------------PQGVLSVDSA--MPMVLHLLAPLAAKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLFDSRFRVIASPEYLAKHGTPQSTEELAGHQCLGFTEPGSLNTWAVLDAQGNPYKISPHFTASSGEILRSLCLSGCGIVCLSDFLVDNDIAEGKLIPLLAEQTSDKTHPFNAVYYSDKAVNLRLRVFLDFLVEEL--------

General information: TITO was launched using: RESULT: Template: 3HHF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 966 -22776 -23.58 -114.45 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -23.58 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.331

(partial model without unconserved sides chains): PDB file : Tito_3HHF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3HHF-query.scw PDB file : Tito_Scwrl_3HHF.pdb: