Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKISLLGSGRVAFHLAKALLAQGHHIAQVYARDFEKTKKFAEQI----------Q-----------AKA-CQS-LQEFQPTDLIILAVSDS--AITELATQVHELF-PKMLMVHTSGSTDIQVISQVH---EKAGVFYPLQTFSLERDVDWKATPLFVEA--TNKDDLVILSDLANSLSKRVYQYTSKQRLTLHLAAVFACNFSNYCFDMAKQVVDAEQVDFGLLYPLILETAKKATENDPKQMQTGPAMRGDQNILVMHQSLLAQANRDDLKQVYQLLSDGIVKRHHSS 4PZC Chain:C ((4-247)) RTVGIVGAGTMGNGIAQACAVVGLNVV-MVDISDAAVQKGVATVASSLDRLIKKEKLTEADKASALARIKGSTSYDDLKATDIVIEAATENYDLKVKILKQIDGIVGENVIIASNTSSISITKLAAVTSRADRFIGMHFFNPVPVM------ALVELIRGLQTSDTTHAAVEALSKQLGKYPITVKNSPGF-------VVNRILCPMINEAFCVLGEGLASPEEIDEGMKLGCN---------HPIGPLALADMIGLDTMLAVMEVL-----------------------

General information: TITO was launched using: RESULT: Template: 4PZC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1061 -63037 -59.41 -295.95 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain C : 0.66 3D Compatibility (PKB) : -59.41 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.453

(partial model without unconserved sides chains): PDB file : Tito_4PZC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4PZC-query.scw PDB file : Tito_Scwrl_4PZC.pdb: