Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRDLIVQWLTELTQPFWHSSLSIDQFVTALQETFHMVFFALLFGGIWGFIQGIILLVTRPEGILPNKFIYHLLNPIVNALRSLPFIILLIAVIPLTKLLVGTS-------------------IGTWAAIVPLTIYVGPYMGRLIETSLLEVNEGIIESAQAMGATPWQIISRFILPEARSSLILNLTTATISLIGATAMAGAVGAGG---------------IGDLAISYGYQ---RFDTSVVILTVIVLLLLVQIVQMLGNWLAKMR 3RLF Chain:F ((277-503)) -------------------------FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEA-------LRGKAVYRVLLILPYAVPSFISILIFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLLKAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNGGPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAI---------

General information: TITO was launched using: RESULT: Template: 3RLF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 638 -91111 -142.81 -479.53 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain F : 0.63 3D Compatibility (PKB) : -142.81 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.370

(partial model without unconserved sides chains): PDB file : Tito_3RLF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3RLF-query.scw PDB file : Tito_Scwrl_3RLF.pdb: