Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLRLKCLGGPLYGQEY----SHAGDEF--------LFNDKQSGKQTLYRKQTLDFTPPQDVFVAESISNIIAYNLALQLKK 5MU9 Chain:A ((244-282)) --------GVMLGVNKAGDAAHAYNFTLTKDHADIVFFEPQNGGYLN----------------------------------

General information: TITO was launched using: RESULT: Template: 5MU9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 40 -306 -7.64 -11.31 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : -7.64 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.177

(partial model without unconserved sides chains): PDB file : Tito_5MU9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5MU9-query.scw PDB file : Tito_Scwrl_5MU9.pdb: